GFP Antibody: Biotin
Product Specifications
Gene Name
Green fluorescent protein
Reactivity
Green Fluorescent Protein
Immunogen
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Target
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca (2+) -activated photoprotein aequorin.
Clonality
Monoclonal
Clone
9F9.F9
Conjugation
Biotin
Type
Monoclonal Antibody
Applications
Enzyme-linked immunosorbent assay|Immunohistochemistry|Purification|Western blot
Purification
GFP Biotin Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (OARA01928, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Homology
Wt, rGFP, eGFP
Format
Lyophilized
Reconstitution
2°C to 8°C|-20°C or -80°C
Molecular Weight
455
Notes
Formerly GWB-FFB8C6
NCBI Gene Symbol
GFP
Host or Source
Mouse
Protein Name
Green fluorescent protein
Gene Name URL
GFP
CAS Number
9007-83-4
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