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MEK 1 Antibody (Phospho-Thr29)

Product Specifications

CAS Number

9007-83-4

Gene Aliases

MEL, CFC3, MEK1, MKK1, MAPKK1, PRKMK1

Gene ID

5604

Accession Number

NP_002746

Reactivity

Human, Mouse, Rat, Cow, Dog

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr292 of human MEK 1.

Target

MEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003) . This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002) . Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) . Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB

Purification

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Homology

Cow; Dog; Human; Mouse; Rat

Format

Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Reconstitution

For long term storage -20C is recommended. Stable at -20C for at least 1 year.

Molecular Weight

~45kDa

Notes

Formerly GWB-24ECBF

Specificity

Specific for the ~45k MEK 1 protein phosphorylated at Thr292 in Western blots.

NCBI Gene Symbol

MAP2K1

Host or Source

Rabbit

Protein Name

Dual specificity mitogen-activated protein kinase kinase 1

Gene Name URL

MAP2K1

Nucleotide Accession Number

NM_002755

Frequently Asked Questions

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