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Synaptotagmin Antibody (Phospho-Ser309)

Product Specifications

Gene Aliases

P65

Gene ID

25716

Accession Number

NP_001028852.2

Reactivity

Human, Mouse, Rat, Cow, Dog, Zebrafish

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser309 of synaptotagmin.

Target

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003) . Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003) . Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IHC

Purification

Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Homology

Cow; Dog; Human; Mouse; Rat; Zebrafish

Format

Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Reconstitution

For long term storage -20C is recommended. Stable at -20C for at least 1 year.

Molecular Weight

~60kDa-62kDa

Notes

Formerly GWB-32B970

Specificity

Specific for the ~60k-62k synaptotagmin protein phosphorylated at Ser309.

NCBI Gene Symbol

SYT1

Host or Source

Rabbit

Protein Name

Synaptotagmin-1

Gene Name URL

SYT1

CAS Number

9007-83-4

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