KCC2 Antibody (Phospho-Ser940)
Product Specifications
CAS Number
9007-83-4
Gene Name
Slc12a5 solute carrier family 12 (potassium-chloride transporter), member 5
Gene Aliases
Kcc2
Gene ID
171373
Accession Number
NP_599190.1
Reactivity
Human, Mouse, Rat, Bovine, Canine
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser940 of rat KCC2.
Target
KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it's rate of internalization from the plasma membrane (Lee et al., 2007) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB
Purification
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.25 mg/ml
Format
Liquid.// 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution
For long term storage -80° C is recommended, but shorter term storage at -20° C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20° C.
Molecular Weight
~135 kDa
Specificity
Specific for the ~135k KCC2 protein phosphorylated at Ser940. Immunolabeling of the KCC2 protein band is blocked by the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.
NCBI Gene Symbol
KCC2
Host or Source
Rabbit
Protein Name
Solute carrier family 12 member 5
Gene Name URL
KCC2
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