What is the catalog number for Aquaporin 2 Antibody (Phospho-Ser261)?

The catalog number for Aquaporin 2 Antibody (Phospho-Ser261) is OAPC00158-100UL.

What sizes are available for Aquaporin 2 Antibody (Phospho-Ser261)?

Aquaporin 2 Antibody (Phospho-Ser261) is available in the following size(s): 100 µL.

Is Aquaporin 2 Antibody (Phospho-Ser261) in stock?

Aquaporin 2 Antibody (Phospho-Ser261) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Aquaporin 2 Antibody (Phospho-Ser261)

Product Specifications

CAS Number

9007-83-4

Gene Aliases

AQP-2, aquaporin-2

Gene ID

25386

Accession Number

NP_037041.2

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Canine, Non-human primate

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of rat aquaporin 2.

Target

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007) . A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002) . Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, DB, IHC

Purification

The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.52 mg/ml

Format

Liquid.//10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western blots

Reconstitution

For long term storage -80° C is recommended, but shorter term storage at -20° C is also acceptable as aliquots may be taken without freezing/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20° C.