Dynamin Antibody (Phospho-Ser778)
Product Specifications
CAS Number
9007-83-4
Gene Aliases
Dnm
Gene ID
140694
Accession Number
NP_542420.1
Reactivity
Human, Mouse, Rat, Cow, Dog
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser778 of rat dynamin.
Target
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002) . Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003) . Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB
Purification
Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Homology
Cow; Dog; Human; Mouse; Rat
Format
Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution
For long term storage -20C is recommended. Stable at -20C for at least 1 year.
Molecular Weight
~95kDa
Notes
Formerly GWB-A0A333
Specificity
Specific for the ~95k dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin) . Immunolabeling is blocked by lambda -phosphatase treatment.
NCBI Gene Symbol
DNM1
Host or Source
Sheep
Protein Name
Dynamin-1
Gene Name URL
DNM1
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
![Rabbit Polyclonal Histone H3 [p Thr6] Antibody [DyLight 405]](https://cdn.genprice.uk/genprice/genprice2.png)
MBS6452748-01 0.1 mL
Macrophage Scavenger Receptor Type 1 (MSR Type 1, MSR-1, MSR Type-1) (MaxLight 550)
Ask
View Details 
MBS6452748-02 5x 0.1 mL
Macrophage Scavenger Receptor Type 1 (MSR Type 1, MSR-1, MSR Type-1) (MaxLight 550)
Ask
View Details