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Sptan1 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Spectrin alpha chain, non-erythrocytic 1

Gene Aliases

A2a, IPF, Spna2

Gene ID

64159

Accession Number

NP_741984.2

Reactivity

Human, Rat, Cow

Immunogen

Recombinant construct containing the seventh, eighth and ninth repeats of human alpha II spectrin expressed in and purified from E. Coli.

Target

The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (alpha 1 and alpha 2) and five beta genes (beta 1- beta 5) . Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids (Bennett and Baines 2001) . Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis (Eber and Lux 2004) .

Clonality

Monoclonal

Clone

3D7

Type

Monoclonal Antibody

Applications

WB, IF

Purification

Affinity purified from tissue culture supernatant.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Homology

Cow; Human; Rat

Format

Liquid. 100 ul in PBS + 10 mM sodium azide

Reconstitution

Store at -20C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Molecular Weight

~240kDa

Fragment

1

Specificity

Specific for the ~240 k alpha II spectrin protein.

NCBI Gene Symbol

SPTAN1

Host or Source

Mouse

Protein Name

Spectrin alpha chain, non-erythrocytic 1

Gene Name URL

Sptan1

Nucleotide Accession Number

NM_171983.2

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