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Anti-Clavesin 1/2

Product Specifications

CAS Number

9007-83-4

Gene Aliases

Rlbp1l1, Clavesin-1, RGD1564200

Gene ID

366311

Accession Number

NP_001102439.1

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken from the C-terminus of rat clavesin 1.

Target

Clavesin (clathrin vesicle associated Sec14 protein) is a novel neuron specific protein that has recently been identified and shown to be required for normal morphology of late endosomes and/or lysosomes as lentiviral-mediated knockdown of clavesin in hippocampal neurons causes lysosomal defects (Katoh et al., 2009) . Additionally, upregulation of clavesin in human hepatocellular carcinoma has recently been demonstrated thus making it a useful marker for this disease state (Zhao et al., 2008) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, ICC

Purification

Prepared from serum by affinity purification using a column to which the peptide antigen was coupled.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Homology

Human; Mouse; Rat

Format

Liquid. 100 ul in PBS + 50% glycerol.

Reconstitution

Store at -20C in undiluted aliquots; stable for up at least 1 year after date of receipt. Avoid freeze/thaw cycles.

Molecular Weight

~35kDa

Specificity

Specific for the ~ 35 kDa clavesin 1/2 protein doublet in Western blots of rat brain lysate. Isoform-specific knock down in cultured hippocampal neurons indicates that the lower and upper bands are clavesin 1 and 2, respectively.

NCBI Gene Symbol

CLVS1/2

Host or Source

Rabbit

Protein Name

Clavesin-1

Gene Name URL

CLVS1/2

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