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Anti-Tyrosine Hydroxylase

Product Specifications

CAS Number

9007-83-4

Gene Aliases

The

Gene ID

25085

Accession Number

NP_036872.1

Reactivity

Rat, Most mammals, Some invertebrates

Immunogen

SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.

Target

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999) . TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IF, IHC

Purification

Prepared from rabbit serum by affinity purification using a protein A column and using a column to which the immunogen was coupled.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Homology

Most mammals, Some invertebrates

Format

Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Reconstitution

For long term storage -20C is recommended. Stable at -20C for at least 1 year.

Molecular Weight

~60kDa

Notes

Formerly GWB-8FC223

Specificity

Specific for the ~60k tyrosine hydroxylase protein.

NCBI Gene Symbol

TH

Host or Source

Rabbit

Protein Name

Tyrosine 3-monooxygenase

Gene Name URL

TH

Frequently Asked Questions

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