What is the catalog number for Anti-Neurofilament L (NF-L)?

The catalog number for Anti-Neurofilament L (NF-L) is OAPC00027-100UL.

What sizes are available for Anti-Neurofilament L (NF-L)?

Anti-Neurofilament L (NF-L) is available in the following size(s): 100 µL.

Is Anti-Neurofilament L (NF-L) in stock?

Anti-Neurofilament L (NF-L) is currently made to order / not held in immediate stock. Contact us for lead time.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

Anti-Neurofilament L (NF-L)

Product Specifications

CAS Number

9007-83-4

Gene Aliases

NFL, NF-L, NF68, CMT1F, CMT2E, CMTDIG, PPP1R110

Gene ID

4747

Accession Number

NP_776546.1

Reactivity

Human, Mouse, Rat, Cow, Chicken

Immunogen

Preparation of bovine spinal cord intermediate filaments

Target

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1) . NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IF

Purification

Total IgY fraction.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

Varies by lot. See vial for concentration.

Format

Liquid// with 10mM sodium azide. Adequate amount of material to conduct 10 mini Western blots.

Reconstitution

Store at -20° C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Molecular Weight

~68kDa

Specificity

Specific for the ~68k Neurofilament L protein in Western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.