Anti-Microtubule Associated Protein 2 (MAP2)
Product Specifications
CAS Number
9007-83-4
Gene Aliases
MAP-2, MAP2A, MAP2B, MAP2C
Gene ID
4133
Accession Number
NP_002365
Reactivity
Human, Mouse, Rat, Cow
Target
Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994) . MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau (tau) . MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IF
Purification
The antigen is a recombinant bovine MAP2 expressed in and purified from E. coli. The antibody is total IgY fractionTotal IgY fraction
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
Varies by lot. See vial for concentration.
Format
Liquid.// containing 10 mM NaN3. Adequate amount of material to conduct 10 mini-Western Blots.3
Reconstitution
Store at -20° C; stable for one year. Avoid freeze/thaw cycles.
Molecular Weight
~280kDa
Specificity
Specific for the ~ 280k MAP2 protein in Western blots of rat brain homogenate.
NCBI Gene Symbol
MAP2
Host or Source
Chicken
Protein Name
Microtubule-associated protein 2
Gene Name URL
MAP2
Nucleotide Accession Number
NM_001039538
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