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Vinculin Antibody - C-terminal

Product Specifications

CAS Number

9007-83-4

Gene Name

Vinculin

Gene Aliases

CMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; metavinculin; meta-vinculin; MV; MVCL; vinculin; vinculin isoform meta-VCL.

Gene ID

7414

Accession Number

https://www.ncbi.nlm.nih.gov/protein/NP_003364

Reactivity

Chicken|Human|Mouse|Porcine|Rat

Immunogen

Anti-Vinculin antibody (7489) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Vinculin. The immunogen is located within amino acids 1060-1110 of Vinculin.

Target

Actin filament (F-actin) -binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Homology

Pig (100%), Chicken (100%)

Format

Liquid. Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.

Reconstitution

Store at 4° C for three months; store at -20° C for up to one year.

Molecular Weight

Predicted: 124kD Observed: 124kD

Fragment

IgG

Specificity

Vinculin antibody is human, mouse, and rat specific. At least three isoforms of Vinculin are known to exist.

NCBI Gene Symbol

VCL

Host or Source

Rabbit

Protein Name

Vinculin|vinculin isoform VCL [Homo sapiens]

Gene Name URL

VCL

Nucleotide Accession Number

NM_003373.3

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