Fat Free Antibody - C-terminal

Product Specifications

CAS Number

9007-83-4

Gene Name

VPS51 subunit of GARP complex

Gene Aliases

ANG2; ANG3; another new gene 2 protein; C11orf2; C11orf3; FFR; PCH13; protein fat-free homolog; vacuolar protein sorting 51 homolog; vacuolar protein sorting-associated protein 51 homolog; VPS51, GARP complex subunit.

Gene ID

738

Accession Number

https://www.ncbi.nlm.nih.gov/protein/NP_037397

Reactivity

Bovine|Chicken|Human|Mouse|Rat

Immunogen

Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free. The immunogen is located within amino acids 620 - 670 of Fat Free.

Target

Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN) . The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN (PubMed:20685960) . Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane (PubMed:25799061) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Western blot

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol