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ANKLE2 Antibody - N-terminal

Product Specifications

CAS Number

9007-83-4

Gene Name

Ankyrin repeat and LEM domain containing 2

Gene Aliases

Ankyrin repeat and LEM domain-containing protein 2; KIAA0692; LEM domain containing 7; LEM domain-containing protein 4; Lem4; LEMD7; MCPH16.

Gene ID

23141

Accession Number

https://www.ncbi.nlm.nih.gov/protein/AAH43157

Reactivity

Human|Mouse|Rat

Immunogen

ANKLE2 antibody was raised against a 19 amino acid synthetic peptide near the center of human ANKLE2. The immunogen is located within amino acids 280 - 330 of ANKLE2.

Target

Involved in mitotic nuclear envelope reassembly by promoting dephosphorylation of BAF/BANF1 during mitotic exit. Coordinates the control of BAF/BANF1 dephosphorylation by inhibiting VRK1 kinase and promoting dephosphorylation of BAF/BANF1 by protein phosphatase 2A (PP2A), thereby facilitating nuclear envelope assembly. It is unclear whether it acts as a real PP2A regulatory subunit or whether it is involved in recruitment of the PP2A complex. Involved in brain development (PubMed:25259927) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Western blot

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. ANKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.

Reconstitution

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

Molecular Weight

Predicted: 121, 139, 145 kDa Observed: 121 kDa

Fragment

IgY

NCBI Gene Symbol

ANKLE2

Host or Source

Chicken

Protein Name

ANKLE2 protein [Homo sapiens]|Ankyrin repeat and LEM domain-containing protein 2

Gene Name URL

ANKLE2

Nucleotide Accession Number

NM_015114.2

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