STEAP1 Antibody

STEAP1 Antibody: The six-transmembrane epithelial antigen of prostate 1 (STEAP1) was the first member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. The normal function of STEAP is still uncertain; unlike other members of the STEAP family, STEAP1 does not promote iron or copper reduction or uptake and lacks the FNO-like reductase domain critical for activity. However, its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers. Supporting this is evidence that STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells, suggesting that STEAP1 may a potential target for cancer immunotherapy. At least three isoforms of STEAP1 are known to exist.

Product Specifications

CAS Number

9007-83-4

Gene Name

STEAP family member 1

Gene Aliases

Metalloreductase STEAP1; PRSS24; six transmembrane epithelial antigen of the prostate 1; six-transmembrane epithelial antigen of prostate 1; STEAP; STEAP1 metalloreductase.

Gene ID

26872

Accession Number

https://www.ncbi.nlm.nih.gov/protein/EAL24166

Reactivity

Human|Mouse|Porcine|Rat

Immunogen

STEAP1 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human STEAP1. The immunogen is located within the last 50 amino acids of STEAP1.

Target

Metalloreductase that has the ability to reduce both Fe (3+) to Fe (2+) and Cu (2+) to Cu (1+) . Uses NAD (+) as acceptor (By similarity) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot

Purification

Affinity Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Homology

Pig (94%)

Format

Liquid. STEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Reconstitution

STEAP1 antibody can be stored at 4° C for three months and -20° C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.