BCL2 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Aliases
Bcl-2, PPP1R50
Gene ID
596
Accession Number
NP_000624.2
Reactivity
Human
Immunogen
The immunogen for anti-BCL2 antibody: anti-human BCL2 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human BCL2 amino acids 1-211 purified from E. coli.
Target
BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
Clonality
Monoclonal
Clone
PAT1B5AT
Type
Monoclonal Antibody
Applications
IF, WB
Purification
BCL2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid// PBS (pH 7.4) with 0.1% sodium azide
Reconstitution
For periods up to one month, store at 4C. For periods up to 12 months, store at -20C. Prevent freeze/thaw cycles.
Notes
Formerly GWB-BSP884
Fragment
Mouse IgG2b heavy chain and kappa light chain.
NCBI Gene Symbol
BCL2
Host or Source
Mouse
Protein Name
Apoptosis regulator Bcl-2
Gene Name URL
BCL2
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