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VAMP2 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Aliases

SYB2, VAMP-2, NEDHAHM

Gene ID

6844

Accession Number

NP_055047.2

Reactivity

Human

Immunogen

Anti-human VAMP2 mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human VAMP2 amino acids 1-89 purified from E. coli.

Target

Synaptobrevin 2 which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25.

Clonality

Monoclonal

Clone

P3E5AT

Type

Monoclonal Antibody

Applications

WB

Purification

VAMP2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Liquid. 1mg/ml containing PBS, pH-7.4, and 0.1% Sodium Azide.

Reconstitution

Stability: //12 months at -20C. 1 month at 4C.

Notes

Formerly GWB-BSP964

Fragment

Mouse IgG1 heavy chain and kappa light chain.

NCBI Gene Symbol

VAMP2

Host or Source

Mouse

Protein Name

Vesicle-associated membrane protein 2

Gene Name URL

VAMP2

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