Zika Virus NS1 Biotinylated Antibody
Product Specifications
CAS Number
9007-83-4
Reactivity
Viral
Immunogen
Full length recombinant NS1 protein of Zika virus produced in HEK293 cells.
Target
Zika virus is an emerging disease that is spread by Aedes mosquitoes. It is a member of the flavivirus family, and is structurally closely related to viruses such as Dengue Fever Virus.The NS1 protein is a major non-strucutural protein expressed by the Zika Virus. The NS1 monomer is a glycosylated protein of approximately 45kD, which associates with lipids and forms a homodimer inside infected cells. It is necessary for viral replication, and is also secreted into the extracellular space as a hexameric lipoprotein particle, which is involved in immune evasion and pathogenesis by interacting with components from both the innate and adaptive immune systems, as well as other host factors. NS1 is one of the major antigenic markers for viral infection with Zika.
Clonality
Monoclonal
Clone
D11
Type
Monoclonal Antibody
Purification
Antibody was purified from hybridoma cell culture supernatant by affinity chromatography on protein G.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
Batch dependent. 0.5 mg/ml.
Format
Phosphate Buffered Saline pH 7.4 with 1% Bovine Serum Albumin
Reconstitution
Store at +4C until the expiry shown on the label. Avoid repeated freeze/thaw cycles..
Fragment
IgG1
Specificity
This antibody is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus and Yellow Fever Virus. A small amount of cross-reactivity has been observed with the NS1 protein from West Nile Virus in a direct ELISA (see results below) .
Host or Source
Mouse
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