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Dengue Virus NS1 Antibody

Product Specifications

CAS Number

9007-83-4

Reactivity

Viral

Immunogen

Dengue virus cell lysate.

Target

The NS1 protein is a major non-structural protein expressed by the Dengue Virus. The NS1 monomer is a glycosylated protein of approximately 45kD, which associates with lipids and forms a homodimer inside infected cells. It is necessary for viral replication, and is also secreted into the extracellular space as a hexameric lipoprotein particle, which is involved in immune evasion and pathogenesis by interacting with components from both the innate and adaptive immune systems, as well as other host factors. NS1 is one of the major antigenic markers for viral infection with Dengue.

Clonality

Monoclonal

Clone

DA034

Type

Monoclonal Antibody

Purification

Affinity chromatography on protein A.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

Batch dependent. 1.0mg/ml.

Format

Phosphate Buffered Saline pH 7.4

Reconstitution

Store at +4C for up to three months, or at -20C for longer periods. Avoid repeated freeze/thaw cycles.

Fragment

IgG1

Specificity

This antibody is specific for all four Dengue virus serotypes cross-reactivity with NS1 protein reactivity has been observed with the NS1 protein from Virus in a direct ELISA specific for the NS1 protein of Dengue virus, detecting NS1 from strains. It demonstrates negligible from Zika virus. A small amount of cross Japanese Encephalitis (see results below) . No cross-reactivity is seen with Chikungunya virus E1, E2 or C proteins.

Host or Source

Mouse

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