Dengue Virus NS1 Antibody
Product Specifications
CAS Number
9007-83-4
Reactivity
Viral
Immunogen
Dengue virus cell lysate.
Target
The NS1 protein is a major non-structural protein expressed by the Dengue Virus. The NS1 monomer is a glycosylated protein of approximately 45kD, which associates with lipids and forms a homodimer inside infected cells. It is necessary for viral replication, and is also secreted into the extracellular space as a hexameric lipoprotein particle, which is involved in immune evasion and pathogenesis by interacting with components from both the innate and adaptive immune systems, as well as other host factors. NS1 is one of the major antigenic markers for viral infection with Dengue.
Clonality
Monoclonal
Clone
DA034
Type
Monoclonal Antibody
Purification
Affinity chromatography on protein A.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
Batch dependent. 1.0mg/ml.
Format
Phosphate Buffered Saline pH 7.4
Reconstitution
Store at +4C for up to three months, or at -20C for longer periods. Avoid repeated freeze/thaw cycles.
Fragment
IgG1
Specificity
This antibody is specific for all four Dengue virus serotypes cross-reactivity with NS1 protein reactivity has been observed with the NS1 protein from Virus in a direct ELISA specific for the NS1 protein of Dengue virus, detecting NS1 from strains. It demonstrates negligible from Zika virus. A small amount of cross Japanese Encephalitis (see results below) . No cross-reactivity is seen with Chikungunya virus E1, E2 or C proteins.
Host or Source
Mouse
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