NDC80 Antibody (Phospho-Ser165)

Product Specifications

CAS Number

9007-83-4

Gene Name

NDC80, kinetochore complex component

Gene Aliases

HEC, HEC1, TID3, KNTC2, HsHec1, hsNDC80

Gene ID

10403

Accession Number

NP_006092.1

Reactivity

Human, Mouse, Rat, Kangaroo rat

Immunogen

Synthetic phosphopeptide corresponding to the amino acids around Ser 165 conjugated to KLH

Target

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

ICC, IF, WB

Purification

Affinity purified using antigen

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

Varies by lot. See vial for concentration.

Format

Liquid// 1X PBS

Buffer

1X PBS

Reconstitution

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4° C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze/thaw cycles.