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INS Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Insulin

Gene Aliases

IDDM, ILPR, IRDN, IDDM1, IDDM2, PNDM4, MODY10

Gene ID

3630

Accession Number

NP_000198.1

Reactivity

Human, Mouse, Rat

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human insulin. The exact sequence is proprietary.

Target

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

IHC-P

Purification

Purified by antigen-affinity chromatography.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid// 1XPBS, 20% glycerol (pH 7) with 0.025% Proclin300 added as a preservative.

Buffer

1XPBS, 20% Glycerol (pH7) . 0.025% ProClin 300 was added as a preservative.

Reconstitution

Store as a concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4° C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze/thaw cycles.

Molecular Weight

12 kDa

Fragment

IgG

NCBI Gene Symbol

INS

Host or Source

Rabbit

Protein Name

Insulin

Gene Name URL

INS

Nucleotide Accession Number

NM_000207.2

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