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HIF1A Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Hypoxia inducible factor 1 alpha subunit

Gene Aliases

HIF1, MOP1, PASD8, HIF-1A, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF-1-alpha

Gene ID

3091

Accession Number

NP_001230013.1

Reactivity

Human, Mouse, Rat, Bovine

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.

Target

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT) . This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Polyclonal Antibody

Applications

ICC, IF, IHC-FFPE, IP, WB

Purification

Purified by antigen-affinity chromatography.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.29 mg/ml

Homology

Dog (97%), Pig (95%), Rabbit (95%)

Format

Liquid.// 1XPBS, 1% BSA, 20% Glycerol (pH7) . 0.025% ProClin 300 was added as a preservative.

Reconstitution

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Molecular Weight

93 kDa

Fragment

IgG

NCBI Gene Symbol

HIF1A

Host or Source

Rabbit

Protein Name

Hypoxia-inducible factor 1-alpha

Gene Name URL

HIF1A

Nucleotide Accession Number

NM_001243084.1

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