MANEA Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Mannosidase endo-alpha
Gene Aliases
ENDO, hEndo
Gene ID
79694
Accession Number
NP_078917.2
Reactivity
Human, Mouse
Immunogen
Recombinant protein encompassing a sequence within the center region of human MANEA. The exact sequence is proprietary.
Target
N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]) .[supplied by OMIM, Sep 2008]
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
IHC-P, WB
Purification
Purified by antigen-affinity chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.
Reconstitution
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Fragment
IgG
NCBI Gene Symbol
MANEA
Host or Source
Rabbit
Protein Name
Glycoprotein endo-alpha-1,2-mannosidase
Gene Name URL
MANEA
Nucleotide Accession Number
NM_024641.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
YP-W77663-01 48 Tests
Bovine β2-microglobulin (BMG; β2-MG) ELISA Kit
Sign In for Pricing
View Details YP-W77663-02 96 Tests
Bovine β2-microglobulin (BMG; β2-MG) ELISA Kit
Sign In for Pricing
View Details 
BNC681274-500 1x 500 µL
Cytokeratin 10 (Suprabasal Epithelial Marker) (AE20), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 