ETFA Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Electron transfer flavoprotein alpha subunit
Gene Aliases
EMA, GA2, MADD
Gene ID
2108
Accession Number
NP_000117.1
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary.
Target
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
ICC, IF, IHC-P, IP, WB
Purification
Purified by antigen-affinity chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.91 mg/ml
Format
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.
Reconstitution
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Fragment
IgG
NCBI Gene Symbol
ETFA
Host or Source
Rabbit
Protein Name
Electron transfer flavoprotein subunit alpha, mitochondrial
Gene Name URL
ETFA
Nucleotide Accession Number
NM_000126.3
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