HMGB1 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
High mobility group box 1
Gene Aliases
HMG1, HMG3, HMG-1, SBP-1
Gene ID
3146
Reactivity
Human, Mouse, Rat, Pig
Immunogen
Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
Target
DNA binding proteins that associate with chromatin and have the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V (D) J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) . Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
ICC, IF, IHC, IHC-FFPE, WB
Purification
Purified by antigen-affinity chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.37 mg/ml
Homology
Xenopus laevis (93%), Dog (100%), Rabbit (100%), Chicken (94%), Rhesus Monkey (100%), Bovine (100%)
Format
Liquid// 1XPBS, 20% glycerol (pH 7) with 0.01% Thimerosal added as a preservative
Reconstitution
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Molecular Weight
25 kDa
Fragment
IgG
NCBI Gene Symbol
HMGB1
Host or Source
Rabbit
Gene Name URL
HMGB1
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