MIF Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Macrophage migration inhibitory factor (glycosylation-inhibiting factor)

Gene Aliases

GIF, GLIF, MMIF

Gene ID

4282

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary.

Target

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

IF, ICC, IHC-FFPE, WB

Purification

Purified by antigen-affinity chromatography.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1mg/ml

Homology

Pig (94%), Sheep (91%), Rhesus Monkey (100%), Bovine (91%)

Format

Liquid.// 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.

Reconstitution

Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks) . For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.