BMP4 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Bone morphogenetic protein 4

Gene Aliases

ZYME, BMP2B, OFC11, BMP2B1, MCOPS6

Gene ID

652

Reactivity

Human, Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of human BMP4. The exact sequence is proprietary.

Target

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

IF, IHC-P, WB, ICC

Purification

Purified by antigen-affinity chromatography.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.36 mg/ml

Format

Liquid// 1X PBS, 1% BSA and 20% glycerol (pH 7) with 0.025% Proclin300

Reconstitution

Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4° C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze/thaw cycles.

Molecular Weight

47 kDa

Fragment

IgG

Specificity

This antibody is able to recognize full length BMP4 protein (~55 kDa) and N-terminal propeptide BMP4 protein (~40 kDa) but unable to detect mature form.