RAD18 antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
E3 ubiquitin-protein ligase RAD18
Gene Aliases
RNF73
Gene ID
56852
Accession Number
NP_064550.3
Reactivity
Human, Bovine, Pig
Immunogen
Recombinant protein encompassing a sequence within the center region of human RAD18. The exact sequence is proprietary.
Target
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
ICC, IF, IHC-FFPE
Purification
Purified by antigen-affinity chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.46 mg/ml
Homology
Human (100%), Bovine (80%), Pig (84%)
Format
Liquid.// 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.
Reconstitution
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Molecular Weight
56 kDa
Fragment
IgG
NCBI Gene Symbol
RAD18
Host or Source
Rabbit
Protein Name
E3 ubiquitin-protein ligase RAD18
Gene Name URL
RAD18
Frequently Asked Questions
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