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TRIM21 antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

E3 ubiquitin-protein ligase TRIM21

Gene Aliases

SSA, RO52, SSA1, RNF81, Ro/SSA

Gene ID

6737

Accession Number

NP_003132.2

Reactivity

Human

Immunogen

Recombinant fragment corresponding to a region within amino acids 254 and 475 of SSA1 (Uniprot ID#P19474)

Target

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

ICC, IF, IHC-FFPE, IP, WB

Purification

Purified by antigen-affinity chromatography.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Homology

Cat (86%), Pig (81%), Rhesus Monkey (99%), Bovine (82%)

Format

Liquid.// 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7) . 0.01% Thimerosal was added as a preservative.

Reconstitution

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Molecular Weight

54 kDa

Fragment

IgG

NCBI Gene Symbol

TRIM21

Host or Source

Rabbit

Protein Name

E3 ubiquitin-protein ligase TRIM21

Gene Name URL

TRIM21

Frequently Asked Questions

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