POLR2B antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
DNA-directed RNA polymerase II subunit RPB2
Gene Aliases
RPB2, POL2RB, hRPB140
Gene ID
5431
Accession Number
NP_000929.1
Reactivity
Human, Mouse
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
Target
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
ICC, IF, IHC-P, WB, CHIP
Purification
Purified by antigen-affinity chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.32 mg/ml
Format
Liquid// 0.1M Tris-Glycine, 20% glycerol (pH 7) with 0.01% Thimerosal
Reconstitution
Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4° C. For long-term store at -20° C or below. Avoid multiple-freeze thaw cycles.
Molecular Weight
134 kDa
Fragment
IgG
NCBI Gene Symbol
POLR2B
Host or Source
Rabbit
Protein Name
DNA-directed RNA polymerase II subunit RPB2
Gene Name URL
POLR2B
Frequently Asked Questions
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