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POLR2B antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

DNA-directed RNA polymerase II subunit RPB2

Gene Aliases

RPB2, POL2RB, hRPB140

Gene ID

5431

Accession Number

NP_000929.1

Reactivity

Human, Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Target

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

ICC, IF, IHC-P, WB, CHIP

Purification

Purified by antigen-affinity chromatography.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.32 mg/ml

Format

Liquid// 0.1M Tris-Glycine, 20% glycerol (pH 7) with 0.01% Thimerosal

Reconstitution

Keep as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4° C. For long-term store at -20° C or below. Avoid multiple-freeze thaw cycles.

Molecular Weight

134 kDa

Fragment

IgG

NCBI Gene Symbol

POLR2B

Host or Source

Rabbit

Protein Name

DNA-directed RNA polymerase II subunit RPB2

Gene Name URL

POLR2B

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