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BAX Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Apoptosis regulator BAX

Gene Aliases

BCL2L4

Gene ID

581

Accession Number

NP_001278357.1NP_001278360.1NP_004315.1NP_620116.1NP_620118.1NP_620119.2

Reactivity

Mouse

Immunogen

Synthetic peptide corresponding to amino acids 172 to 199 of the Bax-beta protein

Target

Bax-beta is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the Bax-beta protein before a stop codon is encountered during translation. Although the Bax-beta protein contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain. Bax-beta mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with Bax-alpha in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. Bax-beta mRNA was very abundant in brain and duodenum with almost no Bax-alpha mRNA detected in these tissues

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

WB, IP

Purification

Ammonium Sulfate Precipitation

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid// PBS with 0.08% sodium azide

Reconstitution

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Molecular Weight

21 kDa

Fragment

IgG

NCBI Gene Symbol

BAX

Host or Source

Sheep

Gene Name URL

BAX

Nucleotide Accession Number

NM_001291428.1NM_001291431.1NM_004324.3NM_138761.3NM_138763.3NM_138764.4

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