TNF Antibody
Product Specifications
Gene Name
Tumor necrosis factor
Gene Aliases
APC1 protein; Cachectin; DIF; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNFA; TNF-alpha; TNFSF2; TNLG1F; tumor necrosis factor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha.
Gene ID
7124
Accession Number
NP_000585.2
Reactivity
Human|Non-human Primate|Porcine
Immunogen
Recombinant human TNF-alpha
Target
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208) . Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:22517918) .
Clonality
Monoclonal
Clone
MAb11
Conjugation
R-PE
Type
Monoclonal Antibody
Applications
Flow cytometry
Purification
Purified by size-exclusion chromatography
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
10 ul / test
Format
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Buffer
The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Reconstitution
2°C to 8°C
Fragment
IgG1
Specificity
The mouse monoclonal antibody MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha) .
NCBI Gene Symbol
TNF
Host or Source
Mouse
Protein Name
Tumor necrosis factor
Gene Name URL
TNF
CAS Number
9007-83-4
Nucleotide Accession Number
NM_000594.3
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
NBP2-90749JF646 0.1 mL
Rabbit Monoclonal NAALADase-like 1/NAALADL1 Antibody (026) [Janelia Fluor 646]
Ask
View Details MBS1237070-01 0.02 mg (E-Coli)
Recombinant Yersinia pseudotuberculosis serotype O:3 UPF0042 nucleotide-binding protein YPK_0502 (YPK_0502)
Ask
View Details MBS1237070-02 0.02 mg (Yeast)
Recombinant Yersinia pseudotuberculosis serotype O:3 UPF0042 nucleotide-binding protein YPK_0502 (YPK_0502)
Ask
View Details