SIT1 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Signaling threshold regulating transmembrane adaptor 1
Gene Aliases
Gp30/40; SHP2 interacting transmembrane adaptor; SHP-2 interacting transmembrane adaptor protein; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; signaling threshold-regulating transmembrane adapter 1; SIT; SIT-R; suppression inducing transmembrane adaptor 1; suppression-inducing transmembrane adapter 1.
Gene ID
27240
Accession Number
NP_055265.1
Reactivity
Human
Immunogen
Bacterially produced recombinant intracellular fragment of human SIT.
Target
Negatively regulates TCR (T-cell antigen receptor) -mediated signaling in T-cells. Involved in positive selection of T-cells.
Clonality
Monoclonal
Clone
SIT-01
Conjugation
R-PE
Type
Monoclonal Antibody
Applications
Flow cytometry
Purification
Purified by size-exclusion chromatography
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.1 mg/ml
Format
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Buffer
The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Reconstitution
2°C to 8°C
Fragment
IgG1
Specificity
The antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.
NCBI Gene Symbol
SIT1
Host or Source
Mouse
Protein Name
Signaling threshold-regulating transmembrane adapter 1
Gene Name URL
SIT1
Nucleotide Accession Number
NM_014450.2
Curated Selection
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