SIT1 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Signaling threshold regulating transmembrane adaptor 1

Gene Aliases

Gp30/40; SHP2 interacting transmembrane adaptor; SHP-2 interacting transmembrane adaptor protein; SHP2-interacting transmembrane adapter protein; SHP2-interacting transmembrane adaptor protein; signaling threshold-regulating transmembrane adapter 1; SIT; SIT-R; suppression inducing transmembrane adaptor 1; suppression-inducing transmembrane adapter 1.

Gene ID

27240

Accession Number

NP_055265.1

Reactivity

Human

Immunogen

Bacterially produced recombinant intracellular fragment of human SIT.

Target

Negatively regulates TCR (T-cell antigen receptor) -mediated signaling in T-cells. Involved in positive selection of T-cells.

Clonality

Monoclonal

Clone

SIT-01

Conjugation

R-PE

Type

Monoclonal Antibody

Applications

Flow cytometry

Purification

Purified by size-exclusion chromatography

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.1 mg/ml

Format

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Buffer

The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Reconstitution

2°C to 8°C

Fragment

IgG1

Specificity

The antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with mouse SIT.