PRF1 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Perforin 1
Gene Aliases
Cytolysin; HPLH2; lymphocyte pore-forming protein; P1; perforin 1 (pore forming protein) ; perforin-1; PFP.
Gene ID
5551
Accession Number
NP_001076585.1NP_005032.2
Reactivity
Bovine|Human
Immunogen
Purified granules from human YT lymphoma cell line
Target
Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Clonality
Monoclonal
Clone
DG9
Type
Monoclonal Antibody
Applications
Flow cytometry|Immunocytochemistry|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation
Purification
Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Reconstitution
2°C to 8°C
Molecular Weight
70 kDa
Fragment
IgG2b
Specificity
The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
NCBI Gene Symbol
PRF1
Host or Source
Mouse
Protein Name
Perforin-1
Gene Name URL
PRF1
Nucleotide Accession Number
NM_001083116.1NM_005041.4
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