PAG1 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Phosphoprotein associated with glycosphingolipid microdomains 1

Gene Aliases

Cb; Cbp; Csk-binding protein; F730007C19Rik; Pag; phosphoprotein associated with glycosphingolipid-enriched microdomains 1; phosphoprotein transmembrane adaptor 1; phosphoprotein-associated with GEMs; transmembrane phosphoprotein Cbp.

Gene ID

94212

Accession Number

NP_060910.3

Reactivity

Bovine|Human|Mouse|Rat

Immunogen

C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.

Target

Negatively regulates TCR (T-cell antigen receptor) -mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor) -mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.

Clonality

Monoclonal

Clone

PAG-C1

Type

Monoclonal Antibody

Applications

Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot

Purification

Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Buffer

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Reconstitution

2°C to 8°C

Fragment

IgG2b

Specificity

The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.