PAG1 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Phosphoprotein membrane anchor with glycosphingolipid microdomains 1

Gene Aliases

CBP; Csk-binding protein; PAG; phosphoprotein associated with glycosphingolipid microdomains 1; phosphoprotein associated with glycosphingolipid-enriched microdomains 1; transmembrane adapter protein PAG; transmembrane adaptor protein PAG; transmembrane phosphoprotein Cbp.

Gene ID

55824

Accession Number

NP_060910.3

Reactivity

Human

Immunogen

Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG) .

Target

Negatively regulates TCR (T-cell antigen receptor) -mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor) -mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.

Clonality

Monoclonal

Clone

MEM-255

Type

Monoclonal Antibody

Applications

Flow cytometry|Immunohistochemistry-Paraffin|Western blot

Purification

Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Buffer

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Reconstitution

2°C to 8°C

Fragment

IgG2a

Specificity

The antibody MEM-255 recognizes an epitope (aa 235-280) of Csk-binding protein (Cbp) located in the cytoplasmic domain, also known as protein associated with glycosphingolipid-enriched microdomains (PAG) .