PECAM1 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Platelet and endothelial cell adhesion molecule 1

Gene Aliases

CD31; CD31 antigen; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1.

Gene ID

5175

Accession Number

NP_000433.4

Reactivity

Human

Immunogen

Leukocytes of a patient suffering from LGL-type leukaemia

Target

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308) . Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684) . Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302) . Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308) . Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892) . Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892) . Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896) . Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896) . Induces susceptibility to atherosclerosis (By similarity) .

Clonality

Monoclonal

Clone

MEM-05

Conjugation

Biotin

Type

Monoclonal Antibody

Applications

Flow cytometry|Immunocytochemistry|Immunohistochemistry-Paraffin|Western blot

Purification

Purified by size-exclusion chromatography

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Buffer

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Reconstitution

2°C to 8°C

Fragment

IgG1

Specificity

The antibody MEM-05 reacts with CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.