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Anti-DnaK (E. coli) (8E2/2) Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Chaperone protein DnaK

Gene Aliases

B0014; chaperone protein DnaK; ECK0014; groPAB; groPC; groPF; grpC; grpF; Heat shock 70 kDa protein; Heat shock protein 70; HSP70; seg.

Gene ID

944750

Reactivity

Escherichia coli

Immunogen

E. coli DnaK.

Target

Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.

Clonality

Monoclonal

Clone

8E2/2

Type

Monoclonal Antibody

Applications

Western blot

Purification

Protein G affinity purified.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Format

Liquid.// PBS (ph 7.2), containing 50% glycerol and 0.09% sodium azide.

Reconstitution

Store long term at -20C.

Fragment

IgG1

Specificity

Recognizes bacteria DnaK. Detects a band of ~70kDa by Western blot.

NCBI Gene Symbol

DnaK

Host or Source

Mouse

Protein Name

Chaperone protein DnaK

Gene Name URL

DnaK

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