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Perforin Antibody - PE Conjugated

Product Specifications

CAS Number

9007-83-4

Gene Name

Perforin 1

Gene Aliases

P1, PFP, HPLH2

Gene ID

5551

Accession Number

NP_001076585.1

Reactivity

Human

Target

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9) . Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein.

Clonality

Monoclonal

Clone

B-D48

Conjugation

PE: Phycoerythrin

Type

Monoclonal Antibody

Applications

FC

Purification

Ion exchange chromatography

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

100 tests/1mL

Format

Lyophilized

Buffer

Phosphate-buffered saline with 5% BSA and 0.09% sodium azide

Reconstitution

Reconstitute with 1 mL deionised water. Use 10 uL to label 106 cells or 100 uL of whole blood. Stable at +2-8C for six months after reconstitution. DO NOT FREEZE

Molecular Weight

61 kDa

Fragment

Mouse IgG1

Specificity

Recognizes human Perforin, a 70 kDa protein

NCBI Gene Symbol

Perforin

Host or Source

Mouse

Protein Name

Perforin-1

Gene Name URL

Perforin

Nucleotide Accession Number

NM_001083116.1

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