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CD25 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Interleukin 2 receptor subunit alpha

Gene Aliases

P55, CD25, IL2R, IMD41, TCGFR, IDDM10

Gene ID

3559

Accession Number

NP_000408.1

Reactivity

Human

Target

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

Clonality

Monoclonal

Clone

B-F2

Type

Monoclonal Antibody

Purification

Ion exchange chromatography

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.1 mg/mL

Format

Lyophilized

Buffer

Phosphate-buffered saline. With 1% BSA and 0.09% Sodium Azide*.

Reconstitution

Store at +2-8C for 12 months. For longer storage, freeze aliquots at -20C

Molecular Weight

31 kDa

Fragment

IgG1

Specificity

Recognises the Interleukin-2 Receptor alpha chain (IL-2Ra), a 55 kDa protein

NCBI Gene Symbol

CD25

Host or Source

Mouse

Protein Name

Interleukin-2 receptor subunit alpha

Gene Name URL

CD25

Nucleotide Accession Number

NM_000417.2

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