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CD19 Antibody

CD19 Antibody

Product Specifications

Product Name Alternative

B-lymphocyte antigen CD19; Differentiation antigen CD19; B-lymphocyte surface antigen B4; B4; CD19 molecule; CD19 antigen

UniProt

P25918

Reactivity

Canine, Mouse

Immunogen

This antibody was raised by immunising CD19-deficient mice with a cell line of mCD19+ mouse pre-B cells.

Target

CD19

Clonality

Monoclonal

Clone

MB19-1

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Concentration

1 mg/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This clone can be used to detect and analyze the presence of the CD19 transmembrane protein. The specificity towards the protein has been confirmed using ELISA and immunofluorescence (Sato et al., 1996; PMID: 8906812) . Additionally, the MB19-1 antibody has been used in several flow cytometry assays. One group used this antibody to determine the differences in CD19 distribution between cells of young and old mice (Yamamoto et al., 2018; PMID: 29625072) . Another group used it to differentiate eosinophils from other leukocytes (Vanella et al., 2017; PMID: 27043413) . A final group used flow cytometry to determine the fraction of B cells cells in a sample with this antibody (Bedaret et al., 2009; PMID: 19907079) .

Tested Applications

ELISA, FC, IF

Host or Source

Mouse

Preservative

PBS with 0.02% Proclin 300.

Isotype

IgA

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