PSME3 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Proteasome activator subunit 3

Gene Aliases

11S regulator complex gamma subunit;11S regulator complex subunit gamma; activator of multicatalytic protease subunit 3; epididymis secretory protein Li 283; HEL-S-283; Ki; Ki antigen; Ki nuclear autoantigen; PA28 gamma variant 5; PA28G; PA28gamma; PA28-gamma; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) ; proteasome activator 28 subunit gamma; proteasome activator 28-gamma; proteasome activator complex subunit 3; REG gamma-3; REG-GAMMA.

Gene ID

10197

Accession Number

NP_005780.2

Reactivity

Human|Mouse|Porcine

Immunogen

Recombinant PSME3 (Val7 ~ Tyr254) expressed in E.coli.

Target

Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

Purification

Affinity Purified; Protein A Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

1 mg/ml

Format

Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Reconstitution

2°C to 8°C|-20°C

Fragment

IgG

Specificity

The antibody is a rabbit polyclonal antibody raised against PSME3. It has been selected for its ability to recognize PSME3 in immunohistochemical staining and western blotting.