CD274 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

CD274 antigen

Gene Aliases

A530045L16Rik; B7 homolog 1; B7h1; PD-; PDCD1 ligand 1; Pdcd1l; Pdcd1l1; Pdcd1lg1; Pdl1; programmed cell death 1 ligand 1.

Gene ID

60533

Accession Number

NP_068693.1

Reactivity

Mouse

Immunogen

Recombinant PDL (Leu48 - Thr290) expressed in E.coli

Target

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11238124) . The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity) . May costimulate helper T-cell subsets through a yet unknown activating receptor (PubMed:11015443, PubMed:12719480) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

Purification

Affinity Purified; Protein A Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Format

Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Reconstitution

2°C to 8°C|-20°C

Fragment

IgG

Specificity

The antibody is a rabbit polyclonal antibody raised against PDCD1LG1. It has been selected for its ability to recognize PDCD1LG1 in immunohistochemical staining and western blotting.