POU5F1 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
POU domain, class 5, transcription factor 1
Gene Aliases
NF-A3; Oct; Oct-; Oct3; Oct-3; Oct3/; Oct-3/; Oct3/4; Oct-3/4; Oct4; Oct-4; octamer-binding protein 3; octamer-binding protein 4; octamer-binding transcription factor 3; octamer-binding transcription factor 4 alternative; Otf; Otf-; Otf3; Otf3-; Otf-3; Otf3g; Otf3-rs7; Otf4; Otf-4; POU domain class 5 transcription factor 1 transcript variant 2 oct4b'; POU domain class 5 transcription factor 1 transcript variant 3 oct4b; POU domain, class 5, transcription factor 1.
Gene ID
18999
Accession Number
NP_038661.2
Reactivity
Mouse
Immunogen
Range: Ser129 - Asn352
Target
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:1972777, PubMed:1690859, PubMed:1967980, PubMed:17525163, PubMed:23376973) . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:17097055, PubMed:17496161, PubMed:19740739) . Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1972777, PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:19740739, PubMed:29153991, PubMed:23376973) .
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
Purification
Affinity Purified; Protein A Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.32 mg/ml
Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Reconstitution
2°C to 8°C|-20°C
Molecular Weight
38 kDa
Fragment
IgG
Specificity
The antibody is a rabbit polyclonal antibody raised against OCT4. It has been selected for its ability to recognize OCT4 in immunohistochemical staining and western blotting.
NCBI Gene Symbol
Pou5f1
Host or Source
Rabbit
Protein Name
POU domain, class 5, transcription factor 1
Gene Name URL
Pou5f1
Nucleotide Accession Number
NM_013633
Curated Selection
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