MGEA5 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

O-GlcNAcase

Gene Aliases

Beta-hexosaminidase; beta-N-acetylglucosaminidase; beta-N-acetylhexosaminidase; bifunctional protein NCOAT; hyaluronidase in meningioma; MEA5; meningioma expressed antigen 5 (hyaluronidase) ; meningioma-expressed antigen 5; MGEA5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; protein O-GlcNAcase.

Gene ID

10724

Accession Number

NP_001135906.1

Reactivity

Human|Mouse

Immunogen

Range: Ser6 - Lys240

Target

Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210) . Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

Purification

Affinity Purified; Protein A Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Format

Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Reconstitution

2°C to 8°C|-20°C

Molecular Weight

76 kDa

Fragment

IgG

Specificity

The antibody is a rabbit polyclonal antibody raised against NAGase. It has been selected for its ability to recognize NAGase in immunohistochemical staining and western blotting.