AST Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Glutamic-oxaloacetic transaminase 1

Gene Aliases

Aspartate aminotransferase 1; aspartate aminotransferase, cytoplasmic; cAspAT; cCAT; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; Glutamate oxaloacetate transaminase 1; glutamate oxaloacetate transaminase 1, soluble; transaminase A.

Gene ID

281206

Accession Number

NP_803468.1

Reactivity

Bovine|Human|Mouse|Rat

Immunogen

Range: Ala2 - Gln413

Target

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H (2) S via the action of 3-mercaptopyruvate sulfurtransferase (3MST) . Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain (By similarity) .

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

Purification

Affinity Purified; Protein A Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Format

Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Reconstitution

2°C to 8°C|-20°C

Molecular Weight

46 kDa

Fragment

IgG

Specificity

The antibody is a rabbit polyclonal antibody raised against AST. It has been selected for its ability to recognize AST in immunohistochemical staining and western blotting.