ASCL1 Antibody
Product Specifications
CAS Number
9007-83-4
Gene Name
Achaete-scute family bHLH transcription factor 1
Gene Aliases
Achaete scute protein; achaete-scute complex homolog 1; achaete-scute complex-like 1; achaete-scute homolog 1; ASH1; ASH-1; bHLHa46; class A basic helix-loop-helix protein 46; HASH1; MASH1.
Gene ID
429
Accession Number
NP_004307.2
Reactivity
Human
Immunogen
Recombinant ASCL1 (Lys84~Phe326) expressed in E.coli
Target
Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation.
Clonality
Polyclonal
Type
Polyclonal Antibody
Source
Polyclonal antibody preparation
Applications
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
Purification
Affinity Purified; Protein A Purified
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Concentration
0.5 mg/ml
Format
Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Reconstitution
2°C to 8°C|-20°C
Fragment
IgG
Specificity
The antibody is a rabbit polyclonal antibody raised against ASCL1. It has been selected for its ability to recognize ASCL1 in immunohistochemical staining and western blotting.
NCBI Gene Symbol
ASCL1
Host or Source
Rabbit
Protein Name
Achaete-scute homolog 1
Gene Name URL
ASCL1
Nucleotide Accession Number
NM_004316
Curated Selection
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