ALOX12 Antibody

Product Specifications

CAS Number

9007-83-4

Gene Name

Arachidonate 12-lipoxygenase, 12S type

Gene Aliases

12-LOX;12S-lipoxygenase;12S-LOX; arachidonate (12S) -lipoxygenase; arachidonate (15S) -lipoxygenase; Arachidonate 12-lipoxygenase, 12S-type; arachidonate 15-lipoxygenase,15S-type; linoleate 13S-lipoxygenase; lipoxin synthase 12-LO; LOG12; platelet 12-LOX; platelet-type 12-lipoxygenase; platelet-type lipoxygenase 12; polyunsaturated fatty acid lipoxygenase ALOX12.

Gene ID

239

Accession Number

NP_000688.2

Reactivity

Human

Immunogen

Range: Phe352 - Cys656

Target

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S) -hydroperoxyeicosatetraenoic acid/ (12S) -HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S) -HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.

Clonality

Polyclonal

Type

Polyclonal Antibody

Applications

Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot

Purification

Affinity Purified; Protein A Purified

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Concentration

0.5 mg/ml

Format

Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Reconstitution

2°C to 8°C|-20°C

Molecular Weight

76 kDa

Fragment

IgG

Specificity

The antibody is a rabbit polyclonal antibody raised against ALOX12. It has been selected for its ability to recognize ALOX12 in immunohistochemical staining and western blotting.